Application

Anti-Rab7 antibody can be used in indirect immunofluorescence and immunoblotting.

Anti-Rab7 antibody produced in rabbit has been used in confocal microscopy.

Biochem/physiol Actions

Rab family of small guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Conversion from one state to the other is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are RILP (Rab-interacting lysosome protein) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rabring7 (Rab7- interacting ring finger protein),(10) and the hVPS34/p150 complex. Anti-Rab7 may be used as a marker for late endosomes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ras-related protein Rab-7 (Rab7) is a member of the Rab family of small guanosine triphosphatases (GTPases).

Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Immunogen

Synthetic peptide corresponding to amino acid residues of human Rab7 with C-terminal added cysteine, conjugated to KLH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti- Rab7 recognizes human, mouse and rat Rab7.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.